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Recombinant human YBX1 protein

YBX1, also known as Y box binding protein 1, associates with the selenocysteine insertion sequence and functions in mammalian selenoprotein translation. This protein is a potential drug target in cancer therapy. It helps the replication of adenovirus type 5, a commonly used vector in gene therapy. Thus, YBX1 can cause an oncolytic effect in YBX1 positive cancer cells treated with adenoviruses. Recombinant human YBX1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Nuclease-sensitive element-binding protein 1, BP-8, CSDA2, CSDB, DBPB, MDR-NF1, NSEP-1, NSEP1, YB-1, YB1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

38.3 kDa (347aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Additionnal Information

YBX1, Nuclease-sensitive element-binding protein 1, BP-8, CSDA2, CSDB, DBPB, MDR-NF1, NSEP-1, NSEP1, YB-1, YB1, ATGP2859-10 µg, ATGP2859-20 µg, ATGP2859-50 µg, ATGP2859-100 µg, ATGP2859-250 µg, ATGP2859-500 µg, ATGP2859-1 mg, ATGP2859-10, ATGP2859-20, ATGP2859-50, ATGP2859-100, ATGP2859-250, ATGP2859-500, ATGP2859-1

References & Citations

Shen Q. et al. (2006) J Cell Physiol. 207:775-783.; ; Chernukhin IV. et al. (2000) J Biol Chem. 275:29915-29921

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_004550.2

Uniprot Accession Number

P67809

Species

Human

AA Sequence

MSSEAET QQPPAAPPAA PALSAADTKP GTTGSGAGSG GPGGLTSAAP AGGDKKVIAT KVLGTVKWFN VRNGYGFINR NDTKEDVFVH QTAIKKNNPR KYLRSVGDGE TVEFDVVEGE KGAEAANVTG PGGVPVQGSK YAADRNHYRR YPRRRGPPRN YQQNYQNSES GEKNEGSESA PEGQAQQRRP YRRRRFPPYY MRRPYGRRPQ YSNPPVQGEV MEGADNQGAG EQGRPVRQNM YRGYRPRFRR GPPRQRQPRE DGNEEDKENQ GDETQGQQPP QRRYRRNFNY RRRRPENPKP QDGKETKAAD PPAENSSAPE AEQGGAE

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