Recombinant human HERPUD1 protein

Homocysteine-responsive endoplasmic reticulum-resident ubiquitin-like domain member 1 protein, also known as HERPuD1, is a 391 amino acid multi-pass membrane protein that localizes to the ER and contains one N-terminal ubiquitin-like domain. Widely expressed with highest expression in the brain, HERP is a component of the ERAD system and, via its ubiquitin-like domain, is thought to be involved in the destruction of misfolded proteins. Recombinant human HERPuD1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Homocysteine-responsive endoplasmic reticulum-resident ubiquitin-like domain member 1 protein, HERP, Mif1, SuP, Methyl methanesulfonate (MMF) -inducible fragment protein 1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

31.6 kDa (286aa) confirmed by MALDI-TOF

Additionnal Information

SuP, MMF inducible fragment protein 1, Mif1, Mif 1, Methyl methanesulfonate inducible fragment protein 1, Methyl methanesulfonate (MMF) -inducible fragment protein 1, Homocysteine-responsive endoplasmic reticulum-resident ubiquitin-like domain member 1 protein, Homocysteine responsive endoplasmic reticulum resident ubiquitin like domain member 1 protein, HERP, ATGP2851-10 µg, ATGP2851-20 µg, ATGP2851-50 µg, ATGP2851-100 µg, ATGP2851-250 µg, ATGP2851-500 µg, ATGP2851-1 mg, ATGP2851-010, ATGP2851-020, ATGP2851-050, ATGP2851-100, ATGP2851-250, ATGP2851-500, ATGP2851-01M

References & Citations

Schulze A., et al. (2005) J Mol Biol. 354: 1021-1027.; ; Liang G., et al. (2006) Mol Cell Biol. 26: 7999-8010

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Ubiquitination

NCBI Accession Number

NP_055500

Uniprot Accession Number

Q15011

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMESETEP EPVTLLVKSP NQRHRDLELS GDRGWSVGHL KAHLSRVYPE RPRPEDQRLI YSGKLLLDHQ CLRDLLPKQE KRHVLHLVCN VKSPSKMPEI NAKVAESTEE PAGSNRGQYP EDSSSDGLRQ REVLRNLSSP GWENISRPEA AQQAFQGLGP GFSGYTPYGW LQLSWFQQIY ARQYYMQYLA ATAASGAFVP PPSAQEIPVV SAPAPAPIHN QFPAENQPAN QNAAPQVVVN PGANQNLRMN AQGGPIVEED DEINRD