Recombinant human IL-12 p70 protein
IL12 is a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. It is a disulfide-linked heterodimer composed of the 40 kD cytokine receptor like subunit and a 35 kD subunit. This cytokine is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. IL12 has been found to be important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen. Recombinant human IL12 protein, fused to His-tag at C-terminus, was expressed in insect cells using baculovirus expression system and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Human IL12 stimulates IFN-g secretion of the NK-92 human natural killer cells. The ED50 range ≤1 ng/mL.
Product Name Alternative
Expression System
Baculovirus
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Bioactivity
Concentration
0.5 mg/mL (determined by absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Bioactivity
The activity is determined by the IFN-g ELISA in a using NK-92 human natural killer cells. The ED50 range ≤1 ng/mL.
Molecular Weight
Additionnal Information
References & Citations
Barton J.L., et al. (2000) Eur. J. Immunol. 30:3299-3308; ; Bazan J.F., et al. (2001) J. Immunol. 167:1440-1446
Other References
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Hormones & Cytokines
NCBI Accession Number
NP_002178 (p40) AAK84425 (p35)
Uniprot Accession Number
P29460 (p40) /P29459 (p35)
Species
Human
N Terminal Sequence
Met1
Frequently Asked Questions
Explore Other Products
Browse additional items from our catalog
Cytochrome C Recombinant Rabbit mAb, Cy7 conjugated
