Recombinant human IGSF4A/CADM1 protein

CADM1 mediates homophilic cell-cell adhesion in a Ca2+-independent manner and also mediates heterophilic cell-cell adhesion with CADM3 and PVRL3 in a Ca2+-independent manner. This protein acts as a tumor suppressor in non-small-cell lung cancer (NSCLC) cells. Interaction with CRTAM promotes natural killer (NK) cell cytotoxicity and interferon-gamma (IFN-gamma) secretion by CD8+ cells in vitro as well as NK cell-mediated rejection of tumors expressing CADM3 in vivo. Recombinant human CADM1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Cell adhesion molecule 1, Immunoglobulin superfamily member 4, IgSF4, Nectin-like protein 2, NECL-2, Spermatogenic immunoglobulin superfamily, SgIgSF, Synaptic cell adhesion molecule, SynCAM, Tumor suppressor in lung cancer 1, TSLC-1, ST17, BL2, IGSF4A, SYNCAM1, RA175

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

39.4 kDa (353aa) confirmed by MALDI-TOF

Additionnal Information

IGSF4A, SynCAM1, Cell adhesion molecule 1, BL2, IGSF4, IGSF4A, Necl-2, NECL2, RA175, sgIGSF, ST17, sTSLC-1, SYNCAM, synCAM1, TSLC1, ATGP2840-10 µg, ATGP2840-20 µg, ATGP2840-50 µg, ATGP2840-100 µg, ATGP2840-250 µg, ATGP2840-500 µg, ATGP2840-1 mg, ATGP2840-10, ATGP2840-20, ATGP2840-50, ATGP2840-100, ATGP2840-250, ATGP2840-500, ATGP2840-1

References & Citations

Barton C.H., et al. (1988) Development 104:165-173.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol, 1mM DTT

Scientific Category

Neuroscience

NCBI Accession Number

NP_055148

Uniprot Accession Number

Q9BY67

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSQNLFTKD VTVIEGEVAT ISCQVNKSDD SVIQLLNPNR QTIYFRDFRP LKDSRFQLLN FSSSELKVSL TNVSISDEGR YFCQLYTDPP QESYTTITVL VPPRNLMIDI QKDTAVEGEE IEVNCTAMAS KPATTIRWFK GNTELKGKSE VEEWSDMYTV TSQLMLKVHK EDDGVPVICQ VEHPAVTGNL QTQRYLEVQY KPQVHIQMTY PLQGLTREGD ALELTCEAIG KPQPVMVTWV RVDDEMPQHA VLSGPNLFIN NLNKTDNGTY RCEASNIVGK AHSDYMLYVY DPPTTIPPPT TTTTTTTTTT TTILTIITDS RAGEEGSIRA VDH