Recombinant human eEF-2K/EEF2K protein
Eukaryotic elongation factor-2 kinase, also known as EEF2K, is threonine kinase that regulates protein synthesis by controlling the rate of peptide chain elongation. upon activation by a variety of upstream kinases including AMPK or TRPM7, phosphorylates the elongation factor EEF2 at a single site, renders it unable to bind ribosomes and thus inactive. Recombinant human EEF2K protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
84.6 kDa (748aa)
Additionnal Information
References & Citations
Ryazanov A.G., et al. (1997) Proc. Natl. Acad. Sci. u.S.A. 94:4884-4889; ; Knebel A., et al. (2001) EMBO J. 20:4360-4369
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) 10% glycerol
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
NP_037434.1
Uniprot Accession Number
O00418
Species
Human
AA Sequence
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