Recombinant human GLTPD1 protein

GLTPD1 mediates the intracellular transfer of ceramide-1-phosphate between organelle membranes and the cell membrane. This protein is required for normal structure of the Golgi stacks. GLTPD1 can bind phosphoceramides with a variety of aliphatic chains, but has a preference for lipids with saturated C16:0 or monounsaturated C18:1 aliphatic chains, and is inefficient with phosphoceramides containing lignoceryl (C24:0) . It plays a role in the regulation of the cellular levels of ceramide-1-phosphate, and thereby contributes to the regulation of phospholipase PLA2G4A activity and the release of arachidonic acid. Recombinant human GLTPD1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Glycolipid transfer protein domain-containing protein 1, CPTP, MGC10334, glycolipid transfer protein domain containing 1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

26.8 kDa (237aa) confirmed by MALDI-TOF

Additionnal Information

GLTPD1, Glycolipid transfer protein domain-containing protein 1, CPTP, MGC10334, glycolipid transfer protein domain containing 1, ATGP2830-10 µg, ATGP2830-20 µg, ATGP2830-50 µg, ATGP2830-100 µg, ATGP2830-250 µg, ATGP2830-500 µg, ATGP2830-1 mg, ATGP2830-10, ATGP2830-20, ATGP2830-50, ATGP2830-100, ATGP2830-250, ATGP2830-500, ATGP2830-1

References & Citations

Simanshu D.K., et al. (2013) Nature 500:463-467.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 50% glycerol, 1mM DTT

Scientific Category

Transportation

NCBI Accession Number

NP_001025056

Uniprot Accession Number

Q5TA50

Species

Human

AA Sequence

MDDSETG FNLKVVLVSF KQCLDEKEEV LLDPYIASWK GLVRFLNSLG TIFSFISKDV VSKLRIMERL RGGPQSEHYR SLQAMVAHEL SNRLVDLERR SHHPESGCRT VLRLHRALHW LQLFLEGLRT SPEDARTSAL CADSYNASLA AYHPWVVRRA VTVAFCTLPT REVFLEAMNV GPPEQAVQML GEALPFIQRV YNVSQKLYAE HSLLDLP