Recombinant human Prolyl Oligopeptidase/PREP protein
PREP is a cytosolic prolyl endopeptidase that cleaves peptide bonds on the C-terminal side of prolyl residues within peptides that are up to approximately 30amino acids long. Prolyl endopeptidases have been reported to be involved in the maturation and degradation of peptide hormones and neuropeptides. Recombinant human PREP protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Prolyl endopeptidase, PE, PEP, Post-proline cleaving enzyme, Prolyl endopeptidase
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
83.1 kDa (733aa)
Additionnal Information
References & Citations
Matsuda, T., et al. (2013) Cell Biol. 45 (4), 850-857
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 30% glycerol, 1mM DTT
Scientific Category
Ubiquitination
NCBI Accession Number
NP_002717
Uniprot Accession Number
P48147
Species
Human
AA Sequence
Frequently Asked Questions
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