Recombinant human KLF12 protein

KLF12, as known as Krueppel-like factor 12, is a member of the Kruppel-like zinc finger protein family and can repress expression of the AP-2 alpha gene by binding to a specific site in the AP-2 alpha gene promoter. Activator protein-2 alpha (AP-2 alpha) is a developmentally-regulated transcription factor and important regulator of gene expression during vertebrate development and carcinogenesis. Repression by the encoded protein requires binding with a corepressor, CtBP1. Two transcript variants encoding different isoforms have been found for this gene. cers. Recombinant human KLF12 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

Krueppel-like factor 12, AP-2rep, AP2REP, HSPC122

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

46.6 kDa (425aa)

Additionnal Information

KLF12, Krueppel-like factor 12, AP-2rep, AP2REP, HSPC122, ATGP2825-10 µg, ATGP2825-20 µg, ATGP2825-50 µg, ATGP2825-100 µg, ATGP2825-250 µg, ATGP2825-500 µg, ATGP2825-1 mg, ATGP2825-10, ATGP2825-20, ATGP2825-50, ATGP2825-100, ATGP2825-250, ATGP2825-500, ATGP2825-1

References & Citations

Roth C. et al. (1996) Genomics. 63:384-390.; ; Schuierer M. et al. (2001) J Biol Chem. 276:27944-27949.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.4M Urea

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_009180

Uniprot Accession Number

Q9Y4X4

Species

Human

AA Sequence

MNIHMKR KTIKNINTFE NRMLMLDGMP AVRVKTELLE SEQGSPNVHN YPDMEAVPLL LNNVKGEPPE DSLSVDHFQT QTEPVDLSIN KARTSPTAVS SSPVSMTASA SSPSSTSTSS SSSSRLASSP TVITSVSSAS SSSTVLTPGP LVASASGVGG QQFLHIIHPV PPSSPMNLQS NKLSHVHRIP VVVQSVPVVY TAVRSPGNVN NTIVVPLLED GRGHGKAQMD PRGLSPRQSK SDSDDDDLPN VTLDSVNETG STALSIARAV QEVHPSPVSR VRGNRMNNQK FPCSISPFSI ESTRRQRRSE SPDSRKRRIH RCDFEGCNKV YTKSSHLKAH RRTHTGEKPY KCTWEGCTWK FARSDELTRH YRKHTGVKPF KCADCDRSFS RSDHLALHRR RHMLV