Recombinant human IL-36Ra/IL36RN protein

IL36RN is a member of the interleukin 1 cytokine family. This cytokine was shown to specifically inhibit the activation of NF-kappaB induced by interleukin 1 family, member 6 (IL1F6) . This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. Two alternatively spliced transcript variants encoding the same protein have been reported. Recombinant human IL36RN protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Interleukin-36 receptor antagonist protein, FIL1 delta, IL-1-related protein 3, IL-1RP3, Interleukin-1 HY1, IL-1HY1, Interleukin-1 delta, IL-1 delta, Interleukin-1 family member 5, IL-1F5, Interleukin-1 receptor antagonist homolog 1, IL-1ra homolog 1, Interleukin-1-like protein 1, IL-1L1, FIL1D, IL1F5, IL1HY1, IL1L1, IL1RP3

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Molecular Weight

19.1 kDa (175aa) confirmed by MALDI-TOF

Additionnal Information

IL36Ra, IL1F5, Interleukin 36 receptor antagonist, FIL1, FIL1 (DELTA), FIL1D, IL1F5, IL1HY1, IL1L1, IL1RP3, IL36RA, PSORP, ATGP2819-10 µg, ATGP2819-20 µg, ATGP2819-50 µg, ATGP2819-100 µg, ATGP2819-250 µg, ATGP2819-500 µg, ATGP2819-1 mg, ATGP2819-10, ATGP2819-20, ATGP2819-50, ATGP2819-100, ATGP2819-250, ATGP2819-500, ATGP2819-1

References & Citations

Barton J.L., et al. (2000) Eur. J. Immunol. 30:3299-3308; ; Bazan J.F., et al. (2001) J. Immunol. 167:1440-1446

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) 10% glycerol

Scientific Category

Hormones & Cytokines

NCBI Accession Number

NP_036407

Uniprot Accession Number

Q9UBH0

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MVLSGALCFR MKDSALKVLY LHNNQLLAGG LHAGKVIKGE EISVVPNRWL DASLSPVILG VQGGSQCLSC GVGQEPTLTL EPVNIMELYL GAKESKSFTF YRRDMGLTSS FESAAYPGWF LCTVPEADQP VRLTQLPENG GWNAPITDFY FQQCD