Recombinant human GNGT1 protein
GNGT1, also known as guanine nucleotide-binding protein G (T) subunit gamma-T1, belongs to the G protein gamma family. This protein is involved as a modulator or transducer in various transmembrane signaling systems. It is required for the GTPase activity, for replacement of GDP by GTP, and for G protein- effector interaction. Recombinant human GNGT1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
Guanine nucleotide-binding protein G (T) subunit gamma-T1, GNG1
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
10.6 kDa (94aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Scherer SW. et al. (1996) Genomics. 35:241-243; ; Simonds WF. et al. (1991) J Biol Chem. 266:5363-5366.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.1M NaCl,1mM EDTA
Scientific Category
Signal Transduction
NCBI Accession Number
NP_068774
Uniprot Accession Number
P63211
Species
Human
AA Sequence
MPVINIE DLTEKDKLKM EVDQLKKEVT LERMLVSKCC EEVRDYVEER SGEDPLVKGI PEDKNPFKEL KGGC
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