Recombinant e.coli glk protein
Glk belongs to the bacterial glucokinase family. This protein is not highly important in E. coli as glucose is transported into the cell by the PTS system already as glucose 6-phosphate. Recombinant E. coli glk protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Glucokinase, ECK2384, JW2385
Expression System
E.coli
Antigen Species
E.coli
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
37.1 kDa (344aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Kawai S, Mukai T, et al. (2005) J. Biosci. Bioeng. 99 (4) : 320-30.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol
Scientific Category
Metabolism
NCBI Accession Number
NP_416889
Uniprot Accession Number
P0A6V8
Species
E.coli
AA Sequence
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