Recombinant human IFN-gamma/IFNG protein

IFNG is a member of the type II interferon family. This protein is a soluble cytokine with antiviral, immunoregulatory and anti-tumor properties and is a potent activator of macrophages. Mutations in this gene are associated with aplastic anemia. Recombinant human IFNG protein was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Human IFN-gamma stimulates cytotoxicity of the WiDr human colon colorectal adenocarcinoma cells. The ED50 range ≤ 0.5 ng/mL.

Product Name Alternative

Interferon gamma, Immune interferon, IFG, IFI, IMD69

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE, Bioactivity

Concentration

1 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Bioactivity

Measured in a cytotoxicity assay using WiDr cells. The ED50 range ≤ 0.5 ng/mL.

Molecular Weight

16.3 kDa (139aa) confirmed by MALDI-TOF

Additionnal Information

IFN-g, ATGP2808-10 µg, ATGP2808-20 µg, ATGP2808-50 µg, ATGP2808-100 µg, ATGP2808-250 µg, ATGP2808-500 µg, ATGP2808-1 mg, ATGP2808-10, ATGP2808-20, ATGP2808-50, ATGP2808-100, ATGP2808-250, ATGP2808-500, ATGP2808-1

References & Citations

Dufour C., et al. (2004) Br. J. Haematol. 126:682-685; ; Thiel D.J., et al. (2000) Structure 8:927-936

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Hormones & Cytokines

NCBI Accession Number

NP_000610

Uniprot Accession Number

P01579

Species

Human

AA Sequence

MQDPYVKEAE NLKKYFNAGH SDVADNGTLF LGILKNWKEE SDRKIMQSQI VSFYFKLFKN FKDDQSIQKS VETIKEDMNV KFFNSNKKKR DDFEKLTNYS VTDLNVQRKA IHELIQVMAE LSPAAKTGKR KRSQMLFRG