Recombinant human NCS1 protein

NCS1 is a member of the neuronal calcium sensor gene family, which encode calcium-binding proteins expressed predominantly in neurons. This protein regulates G protein-coupled receptor phosphorylation in a calcium-dependent manner and can substitute for calmodulin. It is associated with secretory granules and modulates synaptic transmission and synaptic plasticity. Multiple transcript variants encoding different isoforms have been found for this gene. Recombinant human NCS1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Neuronal calcium sensor 1 isoform 1, FLuP, FREQ

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

24.3. kDa (213aa) confirmed by MALDI-TOF

Additionnal Information

NCS1, Neuronal calcium sensor 1 isoform 1, FLuP, FREQ, ATGP2807-10 µg, ATGP2807-20 µg, ATGP2807-50 µg, ATGP2807-100 µg, ATGP2807-250 µg, ATGP2807-500 µg, ATGP2807-1 mg, ATGP2807-10, ATGP2807-20, ATGP2807-50, ATGP2807-100, ATGP2807-250, ATGP2807-500, ATGP2807-1

References & Citations

Nakamura T.Y., et al (2001) . Proc. Natl. Acad. Sci. u.S.A. 98:12808-12813; ; Bahi N., et al (2003) . Hum. Mol. Genet. 12:1415-1425

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 30% glycerol, 1mM DTT

Scientific Category

Neuroscience

NCBI Accession Number

NP_055101

Uniprot Accession Number

P62166

Species

Human

AA Sequence

MGKSNSK LKPEVVEELT RKTYFTEKEV QQWYKGFIKD CPSGQLDAAG FQKIYKQFFP FGDPTKFATF VFNVFDENKD GRIEFSEFIQ ALSVTSRGTL DEKLRWAFKL YDLDNDGYIT RNEMLDIVDA IYQMVGNTVE LPEEENTPEK RVDRIFAMMD KNADGKLTLQ EFQEGSKADP SIVQALSLYD GLV