Recombinant human RNGTT protein

RNGTT is a bifunctional mRNA-capping enzyme exhibiting RNA 5'-triphosphatase activity in the N-terminal part and mRNA guanylyltransferase activity in the C-terminal part. It catalyzes the first two steps of cap formation: by removing the gamma-phosphate from the 5'-triphosphate end of nascent mRNA to yield a diphosphate end, and by transferring the gmp moiety of GTP to the 5'-diphosphate terminus. Recombinant human RNGTT protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

MRNA-capping enzyme, RNA guanylyltransferase and 5'-phosphatase, CAP1A, hCAP, HCE, HCE1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

70.9 kDa (620aa)

Additionnal Information

RNGTT, mRNA-capping enzyme, RNA guanylyltransferase and 5'-phosphatase, CAP1A, hCAP, HCE, HCE1, ATGP2805-10 µg, ATGP2805-20 µg, ATGP2805-50 µg, ATGP2805-100 µg, ATGP2805-250 µg, ATGP2805-500 µg, ATGP2805-1 mg, ATGP2805-10, ATGP2805-20, ATGP2805-50, ATGP2805-100, ATGP2805-250, ATGP2805-500, ATGP2805-1

References & Citations

Yue Z., Maldonado E et al. (1997) Proc. Natl. Acad. Sci. u.S.A. 94:12898-12903.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 0.2M NaCl, 40% glycerol, 2mM DTT, 0.1mM PMSF

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_003791

Uniprot Accession Number

O60942

Species

Human

AA Sequence

MAHNKIP PRWLNCPRRG QPVAGRFLPL KTMLGPRYDS QVAEENRFHP SMLSNYLKSL KVKMGLLVDL TNTSRFYDRN DIEKEGIKYI KLQCKGHGEC PTTENTETFI RLCERFNERN PPELIGVHCT HGFNRTGFLI CAFLVEKMDW SIEAAVATFA QARPPGIYKG DYLKELFRRY GDIEEAPPPP LLPDWCFEDD EDEDEDEDGK KESEPGSSAS FGKRRKERLK LGAIFLEGVT VKGVTQVTTQ PKLGEVQQKC HQFCGWEGSG FPGAQPVSMD KQNIKLLDLK PYKVSWKADG TRYMMLIDGT NEVFMIDRDN SVFHVSNLEF PFRKDLRMHL SNTLLDGEMI IDRVNGQAVP RYLIYDIIKF NSQPVGDCDF NVRLQCIERE IISPRHEKMK TGLIDKTQEP FSVRNKPFFD ICTSRKLLEG NFAKEVSHEM DGLIFQPTGK YKPGRCDDIL KWKPPSLNSV DFRLKITRMG GEGLLPQNVG LLYVGGYERP FAQIKVTKEL KQYDNKIIEC KFENNSWVFM RQRTDKSFPN AYNTAMAVCN SISNPVTKEM LFEFIDRCTA ASQGQKRKHH LDPDTELMPP PPPKRPRPLT