Recombinant human MINA protein

MINA is an oxygenase that can act as both a histone lysine demethylase and a ribosomal histidine hydroxylase. This protein is involved in the demethylation of trimethylated 'Lys-9' on histone H3 (H3K9me3), leading to an increase in ribosomal RNA expression. It also catalyzes the hydroxylation of 60S ribosomal protein L27a on 'His-39'. MINA may play an important role in cell growth and survival. It may be involved in ribosome biogenesis, most likely during the assembly process of pre-ribosomal particles. Recombinant human MINA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

MYC induced nuclear antigen, FLJ14393, MDIG, MINA53, NO52

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

54.9 kDa (485aa)

Additionnal Information

MINA, MYC induced nuclear antigen, FLJ14393, MDIG, MINA53, NO52, ATGP2802-10 µg, ATGP2802-20 µg, ATGP2802-50 µg, ATGP2802-100 µg, ATGP2802-250 µg, ATGP2802-500 µg, ATGP2802-1 mg, ATGP2802-10, ATGP2802-20, ATGP2802-50, ATGP2802-100, ATGP2802-250, ATGP2802-500, ATGP2802-1

References & Citations

Strausberg, R.L., et al. (2002) Proc. Natl. Acad. Sci. u.S.A. 99 (26), 16899-16903

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

AAH14928

Uniprot Accession Number

Q8IUF8

Species

Human

AA Sequence

MPKKAKPTGS GKEEGPAPCK QMKLEAAGGP SALNFDSPSS LFESLISPIK TETFFKEFWE QKPLLIQRDD PALATYYGSL FKLTDLKSLC SRGMYYGRDV NVCRCVNGKK KVLNKDGKAH FLQLRKDFDQ KRATIQFHQP QRFKDELWRI QEKLECYFGS LVGSNVYITP AGSQGLPPHY DDVEVFILQL EGEKHWRLYH PTVPLAREYS VEAEERIGRP VHEFMLKPGD LLYFPRGTIH QADTPAGLAH STHVTISTYQ NNSWGDFLLD TISGLVFDTA KEDVELRTGI PRQLLLQVES TTVATRRLSG FLRTLADRLE GTKELLSSDM KKDFIMHRLP PYSAGDGAEL STPGGKLPRL DSVVRLQFKD HIVLTVLPDQ DQSDETQEKM VYIYHSLKNS RETHMMGNEE ETEFHGLRFP LSHLDALKQI WNSPAISVKD LKLTTDEEKE SLVLSLWTEC LIQVV