Recombinant human SMARCA4 protein

SMARCA4 is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. Mutations in this gene cause rhabdoid tumor predisposition syndrome type 2. Multiple transcript variants encoding different isoforms have been found for this gene.. Recombinant human SMARCA4 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

Transcription activator BRG1 isoform A, BAF190, BAF190A, BRG1, hSNF2b, MRD16, RTPS2, SNF2, SNF2L4, SNF2LB, SWI2

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 80% by SDS-PAGE

Molecular Weight

25kDa (211aa)

Additionnal Information

SMARCA4, Transcription activator BRG1 isoform A, BAF190, BAF190A, BRG1, hSNF2b, MRD16, RTPS2, SNF2, SNF2L4, SNF2LB, SWI2, ATGP2799-10 µg, ATGP2799-20 µg, ATGP2799-50 µg, ATGP2799-100 µg, ATGP2799-250 µg, ATGP2799-500 µg, ATGP2799-1 mg, ATGP2799-10, ATGP2799-20, ATGP2799-50, ATGP2799-100, ATGP2799-250, ATGP2799-500, ATGP2799-1

References & Citations

Dykhuizen, E.C, et al. (2013) Nature 497 (7451), 624-627 ; ; Bai, J., et al. (2013) PLoS ONE 8 (3), E59772

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Scientific Category

Neuroscience

NCBI Accession Number

NP_001122321

Uniprot Accession Number

Q9HBD4

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSDPVEILQ EREYRLQARI AHRIQELENL PGSLAGDLRT KATIELKALR LLNFQRQLRQ EVVVCMRRDT ALETALNAKA YKRSKRQSLR EARITEKLEK QQKIEQERKR RQKHQEYLNS ILQHAKDFKE YHRSVTGKIQ KLTKAVATYH ANTEREQKKE NERIEKERMR RLMAEDEEGY RKLIDQKKDK R