Recombinant human GNAQ protein

GNAQ is a guanine nucleotide-binding protein. GNAQ, an alpha subunit in the Gq class, couples a seven-transmembrane domain receptor to activation of phospolipase C-beta. Mutations at this locus have been associated with problems in platelet activation and aggregation. A related pseudogene exists on chromosome 2. Recombinant human GNAQ protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

Guanine nucleotide-binding protein G (q) subunit alpha, CMC1, G-ALPHA-q, GAQ, SWS

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

44.5 kDa (382aa)

Additionnal Information

GNAQ, Guanine nucleotide-binding protein G (q) subunit alpha, CMC1, G-ALPHA-q, GAQ, SWS, ATGP2798-10 µg, ATGP2798-20 µg, ATGP2798-50 µg, ATGP2798-100 µg, ATGP2798-250 µg, ATGP2798-500 µg, ATGP2798-1 mg, ATGP2798-10, ATGP2798-20, ATGP2798-50, ATGP2798-100, ATGP2798-250, ATGP2798-500, ATGP2798-1

References & Citations

Mudhar, H.S, et al. (2013) Br J Ophthalmol 97 (7), 924-928; ; Chishiki, K., et al. (2013) Biochem. Biophys. Res. Commun. 435 (3), 414-419

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M uREA, 10% glycerol

Scientific Category

Cardiovascular

NCBI Accession Number

NP_002063

Uniprot Accession Number

P50148

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMTLESIM ACCLSEEAKE ARRINDEIER QLRRDKRDAR RELKLLLLGT GESGKSTFIK QMRIIHGSGY SDEDKRGFTK LVYQNIFTAM QAMIRAMDTL KIPYKYEHNK AHAQLVREVD VEKVSAFENP YVDAIKSLWN DPGIQECYDR RREYQLSDST KYYLNDLDRV ADPAYLPTQQ DVLRVRVPTT GIIEYPFDLQ SVIFRMVDVG GQRSERRKWI HCFENVTSIM FLVALSEYDQ VLVESDNENR MEESKALFRT IITYPWFQNS SVILFLNKKD LLEEKIMYSH LVDYFPEYDG PQRDAQAARE FILKMFVDLN PDSDKIIYSH FTCATDTENI RFVFAAVKDT ILQLNLKEYN LV