Recombinant human DPY30 protein
As part of the MLL1/MLL complex, DPY30 is involved in the methylation of histone H3 at 'Lys-4', particularly trimethylation. Histone H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. This protein may play some role in histone H3 acetylation. In a teratocarcinoma cell, it plays a crucial role in retinoic acid-induced differentiation along the neural lineage, regulating gene induction and H3 'Lys-4' methylation at key developmental loci. DPY30 may also play an indirect or direct role in endosomal transport. Recombinant human DPY30 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Protein dpy-30 homolog, HDPY-30, Saf19
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
13.6 kDa (122aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Xu Z., et al (2009) . J. Cell Biol. 186:343-353; ; Patel A., et al (2009) . J. Biol. Chem. 284:24242-24256
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 30% glycerol, 2mM DTT
Scientific Category
Other
NCBI Accession Number
NP_115963
Uniprot Accession Number
Q9C005
Species
Human
AA Sequence
MEPEQML EGQTQVAENP HSEYGLTDNV ERIVENEKIN AEKSSKQKVD LQSLPTRAYL DQTVVPILLQ GLAVLAKERP PNPIEFLASY LLKNKAQFED RN
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