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Recombinant human HMGN3 protein

HMGN3, also known as high mobility group nucleosomal binding domain 3, binds thyroid hormone receptor beta, but only in the presence of thyroid hormone. Thyroid hormone receptors are hormone-dependent transcription factors that regulate expression of a variety of specific target genes. It is thought to reduce the compactness of the chromatin fiber in nucleosomes, thereby enhancing transcription from chromatin templates. Recombinant human HMGN3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

High mobility group nucleosomal binding domain 3, PNAS-24, PNAS-25, TRIP7

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by BCA assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

10.8 kDa (100aa) confirmed by MALDI-TOF

Additionnal Information

HMGN3, High mobility group nucleosomal binding domain 3, PNAS-24, PNAS-25, TRIP7, ATGP2796-10 µg, ATGP2796-20 µg, ATGP2796-50 µg, ATGP2796-100 µg, ATGP2796-250 µg, ATGP2796-500 µg, ATGP2796-1 mg, ATGP2796-10, ATGP2796-20, ATGP2796-50, ATGP2796-100, ATGP2796-250, ATGP2796-500, ATGP2796-1

References & Citations

Lee JW. et al. (1995) Mol Endocrinol. 9:243-254; ; West KL. et al. (2001) J Biol Chem. 276:25959-25969.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH7.0) containing 10% glycerol

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_620058

Uniprot Accession Number

Q15651

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMPKRKSP ENTEGKDGSK VTKQEPTRRS ARLSAKPAPP KPEPKPRKTS AKKEPGAKIS RGAKGKKEEK QEAGKEGTEN

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