Recombinant human PELI2 protein

PELI2 is an E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. This protein is involved in the TLR and IL-1 signaling pathways via interaction with the complex containing IRAK kinases and TRAF6. It mediates IL1B-induced IRAK1 'Lys-63'-linked polyubiquitination and possibly 'Lys-48'-linked ubiquitination. PELI2 may be important for LPS- and IL1B-induced MAP3K7-dependent, but not MAP3K3-dependent, NF-kappa-B activation. It can activate the MAP (mitogen activated protein) kinase pathway leading to activation of ELK1. Recombinant human PELI2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

E3 ubiquitin-protein ligase pellino homolog 2, PELI2 - pellino E3, Pellino-2

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 80% by SDS-PAGE

Molecular Weight

48.8 kDa (443aa) confirmed by MALDI-TOF

Additionnal Information

PELI2, E3 ubiquitin-protein ligase pellino homolog 2, PELI2-pellino E3, Pellino-2, ATGP2792-10 µg, ATGP2792-20 µg, ATGP2792-50 µg, ATGP2792-100 µg, ATGP2792-250 µg, ATGP2792-500 µg, ATGP2792-1 mg, ATGP2792-10, ATGP2792-20, ATGP2792-50, ATGP2792-100, ATGP2792-250, ATGP2792-500, ATGP2792-1

References & Citations

Jensen L.E., et al (2003) . FEBS Lett. 545:199-202; ; Strelow A., et al (2003) . FEBS Lett. 547:157-161

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT

Scientific Category

Signal Transduction

NCBI Accession Number

NP_067078

Uniprot Accession Number

Q9HAT8

Species

Human

AA Sequence

MFSPGQE EHCAPNKEPV KYGELVVLGY NGALPNGDRG RRKSRFALYK RPKANGVKPS TVHVISTPQA SKAISCKGQH SISYTLSRNQ TVVVEYTHDK DTDMFQVGRS TESPIDFVVT DTISGSQNTD EAQITQSTIS RFACRIVCDR NEPYTARIFA AGFDSSKNIF LGEKAAKWKN PDGHMDGLTT NGVLVMHPRG GFTEESQPGV WREISVCGDV YTLRETRSAQ QRGKLVESET NVLQDGSLID LCGATLLWRT ADGLFHTPTQ KHIEALRQEI NAARPQCPVG LNTLAFPSIN RKEVVEEKQP WAYLSCGHVH GYHNWGHRSD TEANERECPM CRTVGPYVPL WLGCEAGFYV DAGPPTHAFT PCGHVCSEKS AKYWSQIPLP HGTHAFHAAC PFCATQLVGE QNCIKLIFQG PID