Recombinant human FDFT1 protein

FDFT1 is a membrane-associated enzyme located at a branch point in the mevalonate pathway. The encoded protein is the first specific enzyme in cholesterol biosynthesis, catalyzing the dimerization of two molecules of farnesyl diphosphate in a two-step reaction to form squalene. Recombinant human FDFT1 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

Squalene synthase, DGPT, ERG9, SQS, SS

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

35.4 kDa (306aa)

Additionnal Information

FDFT1, Squalene synthase, DGPT, ERG9, SQS, SS, ATGP2789-10 µg, ATGP2789-20 µg, ATGP2789-50 µg, ATGP2789-100 µg, ATGP2789-250 µg, ATGP2789-500 µg, ATGP2789-1 mg, ATGP2789-10, ATGP2789-20, ATGP2789-50, ATGP2789-100, ATGP2789-250, ATGP2789-500, ATGP2789-1

References & Citations

Tansey TR, Shechter I (2000) Biochim. Biophys. Acta 1529 (1-3) : 49-62.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.4M urea

Scientific Category

Metabolism

NCBI Accession Number

NP_004453

Uniprot Accession Number

P37268

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMEFVKCL GHPEEFYNLV RFRIGGKRKV MPKMDQDSLS SSLKTCYKYL NQTSRSFAAV IQALDGEMRN AVCIFYLVLR ALDTLEDDMT ISVEKKVPLL HNFHSFLYQP DWRFMESKEK DRQVLEDFPT ISLEFRNLAE KYQTVIADIC RRMGIGMAEF LDKHVTSEQE WDKYCHYVAG LVGIGLSRLF SASEFEDPLV GEDTERANSM GLFLQKTNII RDYLEDQQGG REFWPQEVWS RYVKKLGDFA KPENIDLAVQ CLNELITNAL HHIPDVITYL SRLRNQ