Recombinant human GNG13 protein
Heterotrimeric G proteins, which consist of alpha, beta, and gamma subunits, function as signal transducers for the 7-transmembrane-helix G protein-coupled receptors. GNG13 is a gamma subunit that is expressed in taste, retinal, and neuronal tissues and plays a key role in taste transduction Recombinant human GNG13 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Guanine nucleotide binding protein (G protein) gamma 13, Guanine nucleotide binding protein (G protein), gamma 13, G (gamma) 13, h2-35
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 80% by SDS-PAGE
Molecular Weight
10 kDa (87aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Huang L., et al. (1999) Nat. Neurosci. 2:1055-1062; ; Martin J., et al. (2004) Nature. 432:988-994
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 40% glycerol, 2mM DTT, 0.1mM PMSF
Scientific Category
Signal Transduction
NCBI Accession Number
NP_057625
Uniprot Accession Number
Q9P2W3
Species
Human
AA Sequence
MGSSHHHHHH SSGLVPRGSH MGSMEEWDVP QMKKEVESLK YQLAFQREMA SKTIPELLKW IEDGIPKDPF LNPDLMKNNP WVEKGKC
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