Recombinant human MERIT40 protein

BABAM1 is a component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs) . The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. In the BRCA1-A complex, it is required for the complex integrity and its localization at DSBs. Recombinant human BABAM1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

BRISC and BRCA1-A complex member 1, C19orf62, HSPC142, MERIT40, NBA1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

38.9 kDa (352aa) confirmed by MALDI-TOF

Additionnal Information

MERIT40, BRISC and BRCA1-A complex member 1, C19orf62, HSPC142, MERIT40, NBA1, ATGP2780-10 µg, ATGP2780-20 µg, ATGP2780-50 µg, ATGP2780-100 µg, ATGP2780-250 µg, ATGP2780-500 µg, ATGP2780-1 mg, ATGP2780-10, ATGP2780-20, ATGP2780-50, ATGP2780-100, ATGP2780-250, ATGP2780-500, ATGP2780-1

References & Citations

Zheng Y. et al. (2011) Breast Cancer Res Treat. 127:871-877; ; Solyom S. et al. (2010) Breast Cancer Res Treat. 120:165-168.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.1M NaCl

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_001028721

Uniprot Accession Number

Q9NWV8

Species

Human

AA Sequence

MEVAEPS SPTEEEEEEE EHSAEPRPRT RSNPEGAEDR AVGAQASVGS RSEGEGEAAS ADDGSLNTSG AGPKSWQVPP PAPEVQIRTP RVNCPEKVII CLDLSEEMSL PKLESFNGSK TNALNVSQKM IEMFVRTKHK IDKSHEFALV VVNDDTAWLS GLTSDPRELC SCLYDLETAS CSTFNLEGLF SLIQQKTELP VTENVQTIPP PYVVRTILVY SRPPCQPQFS LTEPMKKMFQ CPYFFFDVVY IHNGTEEKEE EMSWKDMFAF MGSLDTKGTS YKYEVALAGP ALELHNCMAK LLAHPLQRPC QSHASYSLLE EEDEAIEVEA TV