Recombinant human DAP protein
DAP is a basic, proline-rich, 15-kD protein. The protein acts as a positive mediator of programmed cell death that is induced by interferon-gamma. Recombinant human DAP protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Death-associated protein, DAP-1, DAP1
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by absorbance at 280 nm)
Purity
> 80% by SDS-PAGE
Molecular Weight
13.6 kDa (125aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Jostins L., et al. (2012) Nature 491 (7422), 119-124; ; Anderson, C.A., et al. (2011) Nat. Genet. 43 (3), 246-252
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT
Scientific Category
Apoptosis
NCBI Accession Number
NP_004385
Uniprot Accession Number
P51397
Species
Human
AA Sequence
MSSPPEG KLETKAGHPP AVKAGGMRIV QKHPHTGDTK EEKDKDDQEW ESPSPPKPTV FISGVIARGD KDFPPAAAQV AHQKPHASMD KHPSPRTQHI QQPRK
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