Recombinant human GFPT1 protein

GFPT1 is the first and rate-limiting enzyme of the hexosamine pathway and controls the flux of glucose into the hexosamine pathway. The product of this gene catalyzes the formation of glucosamine 6-phosphate. Recombinant human GFPT1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Glutamine--fructose-6-phosphate transaminase 1, CMSTA1, GFA, GFAT, GFAT 1, GFAT1, GFAT1m, GFPT, GFPT1L, MSLG

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by BRADFORD assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

43.7 kDa (391aa) confirmed by MALDI-TOF

Additionnal Information

GFPT1, Glutamine--fructose-6-phosphate transaminase 1, CMSTA1, GFA, GFAT, GFAT 1, GFAT1, GFAT1m, GFPT, GFPT1L, MSLG, ATGP2777-10 µg, ATGP2777-20 µg, ATGP2777-50 µg, ATGP2777-100 µg, ATGP2777-250 µg, ATGP2777-500 µg, ATGP2777-1 mg, ATGP2777-10, ATGP2777-20, ATGP2777-50, ATGP2777-100, ATGP2777-250, ATGP2777-500, ATGP2777-1

References & Citations

Selcen, D., et al. (2013) Neurology 81 (4), 370-378 ; ; Huh, S.Y., et al. (2012) Muscle Nerve 46 (4), 600-604

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 50% glycerol, 2mM DTT, 2mM EDTA

Scientific Category

Metabolism

NCBI Accession Number

NP_001231639

Uniprot Accession Number

Q06210

Species

Human

AA Sequence

QQIMKGN FSSFMQKEIF EQPESVVNTM RGRVNFDDYT VNLGGLKDHI KEIQRCRRLI LIACGTSYHA GVATRQVLEE LTELPVMVEL ASDFLDRNTP VFRDDVCFFL SQSGETADTL MGLRYCKERG ALTVGITNTV GSSISRETDC GVHINAGPEI GVASTKAYTS QFVSLVMFAL MMCDDRISMQ ERRKEIMLGL KRLPDLIKEV LSMDDEIQKL ATELYHQKSV LIMGRGYHYA TCLEGALKIK EITYMHSEGI LAGELKHGPL ALVDKLMPVI MIIMRDHTYA KCQNALQQVV ARQGRPVVIC DKEDTETIKN TKRTIKVPHS VDCLQGILSV IPLQLLAFHL AVLRGYDVDF PRNLAKSVTV E