Recombinant human GFPT1 protein
GFPT1 is the first and rate-limiting enzyme of the hexosamine pathway and controls the flux of glucose into the hexosamine pathway. The product of this gene catalyzes the formation of glucosamine 6-phosphate. Recombinant human GFPT1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Glutamine--fructose-6-phosphate transaminase 1, CMSTA1, GFA, GFAT, GFAT 1, GFAT1, GFAT1m, GFPT, GFPT1L, MSLG
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by BRADFORD assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
43.7 kDa (391aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Selcen, D., et al. (2013) Neurology 81 (4), 370-378 ; ; Huh, S.Y., et al. (2012) Muscle Nerve 46 (4), 600-604
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 50% glycerol, 2mM DTT, 2mM EDTA
Scientific Category
Metabolism
NCBI Accession Number
NP_001231639
Uniprot Accession Number
Q06210
Species
Human
AA Sequence
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