Recombinant human PACT protein

PRKRA is a protein kinase activated by double-stranded RNA which mediates the effects of interferon in response to viral infection. Mutations in this gene have been associated with dystonia. Alternative splicing results in multiple transcript variants. Recombinant human PRKRA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Protein kinase interferon-inducible double stranded RNA dependent activator, Protein kinase, interferon-inducible double stranded RNA dependent activator, DYT16, HSD14, PACT, RAX

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 80% by SDS-PAGE

Molecular Weight

36.8 kDa (336aa) confirmed by MALDI-TOF

Additionnal Information

PACT, Protein kinase interferon-inducible double stranded RNA dependent activator, Protein kinase, interferon-inducible double stranded RNA dependent activator, DYT16, HSD14, PACT, RAX, ATGP2775-10 µg, ATGP2775-20 µg, ATGP2775-50 µg, ATGP2775-100 µg, ATGP2775-250 µg, ATGP2775-500 µg, ATGP2775-1 mg, ATGP2775-10, ATGP2775-20, ATGP2775-50, ATGP2775-100, ATGP2775-250, ATGP2775-500, ATGP2775-1

References & Citations

Peters G.A., et al. (2001) Mol. Cell. Biol. 21:1908-1920; ; Peters G.A., et al. (2006) J. Biol. Chem. 281:35129-35136

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 50% glycerol, 2mM DTT, 1mM EDTA

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_003681

Uniprot Accession Number

O75569

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMSQSRHR AEAPPLERED SGTFSLGKMI TAKPGKTPIQ VLHEYGMKTK NIPVYECERS DVQIHVPTFT FRVTVGDITC TGEGTSKKLA KHRAAEAAIN ILKANASICF AVPDPLMPDP SKQPKNQLNP IGSLQELAIH HGWRLPEYTL SQEGGPAHKR EYTTICRLES FMETGKGASK KQAKRNAAEK FLAKFSNISP ENHISLTNVV GHSLGCTWHS LRNSPGEKIN LLKRSLLSIP NTDYIQLLSE IAKEQGFNIT YLDIDELSAN GQYQCLAELS TSPITVCHGS GISCGNAQSD AAHNALQYLK IIAERK