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Recombinant human PPA2 protein

PPA2 is localized to the mitochondrion, is highly similar to members of the inorganic pyrophosphatase (PPase) family, and contains the signature sequence essential for the catalytic activity of PPase. PPases catalyze the hydrolysis of pyrophosphate to inorganic phosphate, which is important for the phosphate metabolism of cells. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. Recombinant human PPA2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Inorganic pyrophosphatase 2 mitochondrial isoform 1, Inorganic pyrophosphatase 2, mitochondrial isoform 1, Pyrophosphatase (inorganic) 2, HSPC124, SID6-306

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

37.1 kDa (325aa) confirmed by MALDI-TOF

Additionnal Information

PPA2, Inorganic pyrophosphatase 2 mitochondrial isoform 1, Inorganic pyrophosphatase 2, mitochondrial isoform 1, Pyrophosphatase (inorganic) 2, HSPC124, SID6-306, ATGP2771-10 µg, ATGP2771-20 µg, ATGP2771-50 µg, ATGP2771-100 µg, ATGP2771-250 µg, ATGP2771-500 µg, ATGP2771-1 mg, ATGP2771-10, ATGP2771-20, ATGP2771-50, ATGP2771-100, ATGP2771-250, ATGP2771-500, ATGP2771-1

References & Citations

Gauthier-Campbell C., Bredt D.S., et al. (2004) Mol. Biol. Cell 15:2205-2217

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Scientific Category

Metabolism

NCBI Accession Number

NP_789845

Uniprot Accession Number

Q9H2U2

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSALYHTEE RGQPCSQNYR LFFKNVTGHY ISPFHDIPLK VNSKEENGIP MKKARNDEYE NLFNMIVEIP RWTNAKMEIA TKEPMNPIKQ YVKDGKLRYV ANIFPYKGYI WNYGTLPQTW EDPHEKDKST NCFGDNDPID VCEIGSKILS CGEVIHVKIL GILALIDEGE TDWKLIAINA NDPEASKFHD IDDVKKFKPG YLEATLNWFR LYKVPDGKPE NQFAFNGEFK NKAFALEVIK STHQCWKALL MKKCNGGAIN CTNVQISDSP FRCTQEEARS LVESVSSSPN KESNEEEQVW HFLGK

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