Recombinant human B9D2 protein

B9D2 contains a B9 domain protein, which are exclusively found in ciliated organisms. This protein is upregulated during mucociliary differentiation, and the encoded protein localizes to basal bodies and cilia. Disrupting expression of this gene results in ciliogenesis defects. Recombinant human B9D2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

B9 domain-containing protein 2, ICIS-1, MKS10, MKSR2

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

21.6 kDa (198aa) confirmed by MALDI-TOF

Additionnal Information

B9D2, B9 domain-containing protein 2, ICIS-1, MKS10, MKSR2, ATGP2768-10 µg, ATGP2768-20 µg, ATGP2768-50 µg, ATGP2768-100 µg, ATGP2768-250 µg, ATGP2768-500 µg, ATGP2768-1 mg, ATGP2768-10, ATGP2768-20, ATGP2768-50, ATGP2768-100, ATGP2768-250, ATGP2768-500, ATGP2768-1

References & Citations

Dowdle W.E., et al. (2011) Am. J. Hum. Genet. 89:94-110; ; Bialas N.J., et al. (2009) J. Cell Sci. 122:611-624

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 50% glycerol, 2mM DTT, 1mM EDTA

Scientific Category

Signal Transduction

NCBI Accession Number

NP_085055

Uniprot Accession Number

Q9BPU9

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMAEVHVI GQIIGASGFS ESSLFCKWGI HTGAAWKLLS GVREGQTQVD TPQIGDMAYW SHPIDLHFAT KGLQGWPRLH FQVWSQDSFG RCQLAGYGFC HVPSSPGTHQ LACPTWRPLG SWREQLARAF VGGGPQLLHG DTIYSGADRY RLHTAAGGTV HLEIGLLLRN FDRYGVEC