Recombinant HCV Core protein

HCV-Core protein packages viral RNA to form a viral nucleocapsid, and promotes virion budding. It modulates viral translation initiation by interacting with HCV IRES and 40S ribosomal subunit and also regulates many host cellular functions such as signaling pathways and apoptosis. HCV-Core prevents the establishment of cellular antiviral state by blocking the interferon-alpha/beta (IFN-alpha/beta) and IFN-gamma signaling pathways and by inducing human STAT1 degradation. Recombinant Hepatitis C virus Core protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

Polyprotein, Human Hepatitis C Virus E2 protein fragment

Expression System

E.coli

Antigen Species

HCV

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

15.7 kDa (140aa)

Additionnal Information

HCV, HCV Core, Polyprotein, Human Hepatitis C Virus E2 protein fragment, ATGP2766-10 µg, ATGP2766-20 µg, ATGP2766-50 µg, ATGP2766-100 µg, ATGP2766-250 µg, ATGP2766-500 µg, ATGP2766-1 mg, ATGP2766-10, ATGP2766-20, ATGP2766-50, ATGP2766-100, ATGP2766-250, ATGP2766-500, ATGP2766-1

References & Citations

Taylor D.R., et al. (2001) J. Virol. 75:1265-1273; ; Kalliampakou K.I., et al. (2015) J. Gen. Virol. 86:1015-1025

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Scientific Category

Virus

NCBI Accession Number

ACE82480

Uniprot Accession Number

B3TKP0

Species

HCV

AA Sequence

MGSSHHHHHH SSGLVPRGSH MSTNPKPQRK TKRNTNRRPQ DVKFPGGGQI VGGVYLLPRR GPRLGVRATR KTSERSQPRG RRQPIPKARR PEGRTWAQPG YPWPLYGNEG CGWAGWLLSP RGSRPSWGPT DPRRRSRNLG