Recombinant human EEF2 protein

EEF2 is a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation. Recombinant human EEF2 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

Elongation factor 2, EEF-2, EF-2, EF2, Eukaryotic translation elongation factor 2, Polypeptidyl-tRNA translocase

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

34.3 kDa (308aa)

Additionnal Information

EEF2, elongation factor 2, EEF-2, EF-2, EF2, ATGP2764-10 µg, ATGP2764-20 µg, ATGP2764-50 µg, ATGP2764-100 µg, ATGP2764-250 µg, ATGP2764-500 µg, ATGP2764-1 mg, ATGP2764-10, ATGP2764-20, ATGP2764-50, ATGP2764-100, ATGP2764-250, ATGP2764-500, ATGP2764-1

References & Citations

Yamashita A., et al (2009) . Genes Dev. 23:1091-1105; ; Olsen J.V., et al (2006) . Cell 127:635-648

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.4M urea

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_001952.1

Uniprot Accession Number

P13639

Species

Human

AA Sequence

DPVVSYR ETVSEESNVL CLSKSPNKHN RLYMKARPFP DGLAEDIDKG EVSARQELKQ RARYLAEKYE WDVAEARKIW CFGPDGTGPN ILTDITKGVQ YLNEIKDSVV AGFQWATKEG ALCEENMRGV RFDVHDVTLH ADAIHRGGGQ IIPTARRCLY ASVLTAQPRL MEPIYLVEIQ CPEQVVGGIY GVLNRKRGHV FEESQVAGTP MFVVKAYLPV NESFGFTADL RSNTGGQAFP QCVFDHWQIL PGDPFDNSSR PSQVVAETRK RKGLKEGIPA LDNFLDKL