Recombinant human EEF2 protein
EEF2 is a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation. Recombinant human EEF2 protein, fused to His-tag at N-terminus, was expressed in E. coli.
Product Specifications
Product Name Alternative
Elongation factor 2, EEF-2, EF-2, EF2, Eukaryotic translation elongation factor 2, Polypeptidyl-tRNA translocase
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Denatured
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
34.3 kDa (308aa)
Additionnal Information
References & Citations
Yamashita A., et al (2009) . Genes Dev. 23:1091-1105; ; Olsen J.V., et al (2006) . Cell 127:635-648
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.4M urea
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
NP_001952.1
Uniprot Accession Number
P13639
Species
Human
AA Sequence
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