Recombinant human EF-1 gamma/EEF1G protein

Elongation factor 1-gamma, also known as EEF1G, is a multi-protein complex that is responsible for the delivery of aminoacyl-tRNAs to the ribosome. This subunit contains an N-terminal glutathione transferase domain, which may be involved in regulating the assembly of multisubunit complexes containing this elongation factor and aminoacyl-tRNA synthetases. Increased expression of EEF1G is associated with pancreatic cancer, suggesting a possible role for EEF1G in the oncogenic transformation process. Recombinant human EEF1G protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Elongation factor 1-gamma, EF1G, GIG35, EF-1-gamma, Elongation factor 1 gamma, GIG35, PRO1608

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

52.5 kDa (460aa)

Additionnal Information

EEF1G, Elongation factor 1-gamma, EF1G, GIG35, EF-1-gamma, Elongation factor 1 gamma, GIG35, PRO1608, ATGP2763-10 µg, ATGP2763-20 µg, ATGP2763-50 µg, ATGP2763-100 µg, ATGP2763-250 µg, ATGP2763-500 µg, ATGP2763-1 mg, ATGP2763-10, ATGP2763-20, ATGP2763-50, ATGP2763-100, ATGP2763-250, ATGP2763-500, ATGP2763-1

References & Citations

Sanders J., et al. (1992) Nucleic Aciads Res. 20: 5907-5910.; ; Koonin E V., et al. (1994) Protein Sci. 3: 2045-2054.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_001395

Uniprot Accession Number

P26641

Species

Human

AA Sequence

MAAGTLY TYPENWRAFK ALIAAQYSGA QVRVLSAPPH FHFGQTNRTP EFLRKFPAGK VPAFEGDDGF CVFESNAIAY YVSNEELRGS TPEAAAQVVQ WVSFADSDIV PPASTWVFPT LGIMHHNKQA TENAKEEVRR ILGLLDAYLK TRTFLVGERV TLADITVVCT LLWLYKQVLE PSFRQAFPNT NRWFLTCINQ PQFRAVLGEV KLCEKMAQFD AKKFAETQPK KDTPRKEKGS REEKQKPQAE RKEEKKAAAP APEEEMDECE QALAAEPKAK DPFAHLPKST FVLDEFKRKY SNEDTLSVAL PYFWEHFDKD GWSLWYSEYR FPEELTQTFM SCNLITGMFQ RLDKLRKNAF ASVILFGTNN SSSISGVWVF RGQELAFPLS PDWQVDYESY TWRKLDPGSE ETQTLVREYF SWEGAFQHVG KAFNQGKIFK