Recombinant human RNA polymerase III subunit RPC10/POLR3K protein
PORL3K is a small essential subunit of RNA polymerase III, the polymerase responsible for synthesizing transfer and small ribosomal RNAs in eukaryotes. The carboxy-terminal domain of this subunit shares a high degree of sequence similarity to the carboxy-terminal domain of an RNA polymerase II elongation factor. This similarity in sequence is supported by functional studies showing that this subunit is required for proper pausing and termination during transcription. Pseudogenes of this gene are found on chromosomes 13 and 17 Recombinant human PORL3K protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
DNA-directed RNA polymerase III subunit RPC10, C11, C11-RNP3, hRPC11, My010, RPC10, RPC11, RPC12.5
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
14.7 kDa (131aa)
Additionnal Information
References & Citations
Johnson, S.S., et al. (2007) Mol. Cell 26 (3), 367-379; ; De Gobbi, M., et al. (2006) Science 312 (5777), 1215-1217;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 50% glycerol, 2mM DTT, 2mM EDTA
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
NP_057394.1
Uniprot Accession Number
Q9Y2Y1
Species
Human
AA Sequence
MLLFCPG CGNGLIVEEG QRCHRFACNT CPYVHNITRK VTNRKYPKLK EVDDVLGGAA AWENVDSTAE SCPKCEHPRA YFMQLQTRSA DEPMTTFYKC CNAQCGHRWR D
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