Recombinant human GARS protein

GARS, also known as glycyl-tRNA synthetase, is one of the aminoacyl-tRNA synthetases that charge tRNAs with their cognate amino acids. This enzyme is an (alpha) 2 dimer which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis. Recombinant human GARS protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Glycyl-tRNA synthetase, CMT2D, DSMAV, GlyRS, HMN5, SMAD1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

30 kDa (270aa) confirmed by MALDI-TOF

Additionnal Information

GARS, Glycyl-tRNA synthetase, CMT2D, DSMAV, GlyRS, HMN5, SMAD1, ATGP2756-10 µg, ATGP2756-20 µg, ATGP2756-50 µg, ATGP2756-100 µg, ATGP2756-250 µg, ATGP2756-500 µg, ATGP2756-1 mg, ATGP2756-10, ATGP2756-20, ATGP2756-50, ATGP2756-100, ATGP2756-250, ATGP2756-500, ATGP2756-1

References & Citations

Guo R.-T., et al. (2009) J. Biol. Chem. 284:28968-28976; ; Xie W., et al. (2007) Proc. Natl. Acad. Sci. u.S.A. 104:9976-9981;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_002038

Uniprot Accession Number

P41250

Species

Human

AA Sequence

PISLPAA ASRSSMDGAG AEEVLAPLRL AVRQQGDLVR KLKEDKAPQV DVDKAVAELK ARKRVLEAKE LALQPKDDIV DRAKMEDTLK RRFFYDQAFA IYGGVSGLYD FGPVGCALKN NIIQTWRQHF IQEEQILEID CTMLTPEPVL KTSGHVDKFA DFMVKDVKNG ECFRADHLLK AHLQKLMSDK KCSVEKKSEM ESVLAQLDNY GQQELADLFV NYNVKSPITG NDLSPPVSFN LMFKTFIGPG