Recombinant human RNA polymerases I and III subunit RPAC1/POLR1C protein

POLR1C is a subunit of both RNA polymerase I and RNA polymerase III complexes. The encoded protein is part of the Pol core element. Defects in this gene have been associated with Treacher Collins syndrome (TCS) . Recombinant human POLR1C protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

DNA-directed RNA polymerases I and III subunit RPAC1, RP3-337H4.4, RPA39, RPA40, RPA5, RPAC1, TCS3

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 80% by SDS-PAGE

Molecular Weight

41.6 kDa (369aa) confirmed by MALDI-TOF

Additionnal Information

POLR1C, DNA-directed RNA polymerases I and III subunit RPAC1, RP3-337H4.4, RPA39, RPA40, RPA5, RPAC1, TCS3, ATGP2753-10 µg, ATGP2753-20 µg, ATGP2753-50 µg, ATGP2753-100 µg, ATGP2753-250 µg, ATGP2753-500 µg, ATGP2753-1 mg, ATGP2753-10, ATGP2753-20, ATGP2753-50, ATGP2753-100, ATGP2753-250, ATGP2753-500, ATGP2753-1

References & Citations

Hu P., et al (2002) . Mol. Cell. Biol. 22:8044-8055; ; Dauwerse J.G., et al (2011) . Nat. Genet. 43:20-22;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_976035

Uniprot Accession Number

O15160

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMAASQAV EEMRSRVVLG EFGVRNVHTT DFPGNYSGYD DAWDQDRFEK NFRVDVVHMD ENSLEFDMVG IDAAIANAFR RILLAEVPTM AVEKVLVYNN TSIVQDEILA HRLGLIPIHA DPRLFEYRNQ GDEEGTEIDT LQFRLQVRCT RNPHAAKDSS DPNELYVNHK VYTRHMTWIP LGNQADLFPE GTIRPVHDDI LIAQLRPGQE IDLLMHCVKG IGKDHAKFSP VATASYRLLP DITLLEPVEG EAAEELSRCF SPGVIEVQEV QGKKVARVAN PRLDTFSREI FRNEKLKKVV RLARVRDHYI FSVESTGVLP PDVLVSEAIK VLMGKCRRFL DELDAVQMD