Recombinant human Vitronectin protein

Vitronectin, also known as VTN, is a member of the pexin family. It is found in serum and tissues and promotes cell adhesion and spreading, inhibits the membrane-damaging effect of the terminal cytolytic complement pathway, and binds to several serpin serine protease inhibitors. Increased expression of VTN, integrins and plasminogen activators has been observed in migrating cells during wound healing. Recombinant human VTN protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

VTN, VN, S-protein, Serum-spreading factor, V75, Vitronectin V65 subunit, Vitronectin V10 subunit, Somatomedin-B, Somatomedin B, Complement S-protein

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

54.7 kDa (482aa)

Additionnal Information

VTN, Vitronectin precursor, V75, VN, VNT, ATGP2741-10 µg, ATGP2741-20 µg, ATGP2741-50 µg, ATGP2741-100 µg, ATGP2741-250 µg, ATGP2741-500 µg, ATGP2741-1 mg, ATGP2741-10, ATGP2741-20, ATGP2741-50, ATGP2741-100, ATGP2741-250, ATGP2741-500, ATGP2741-1

References & Citations

Chain D., et al. (1991) FEBS Lett. 285: 251-256.; ; Cherny R C., et al. (1993) J Biol Chem. 268: 9725-9729.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Scientific Category

Cardiovascular

NCBI Accession Number

NP_000629

Uniprot Accession Number

P04004

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSDQESCKG RCTEGFNVDK KCQCDELCSY YQSCCTDYTA ECKPQVTRGD VFTMPEDEYT VYDDGEEKNN ATVHEQVGGP SLTSDLQAQS KGNPEQTPVL KPEEEAPAPE VGASKPEGID SRPETLHPGR PQPPAEEELC SGKPFDAFTD LKNGSLFAFR GQYCYELDEK AVRPGYPKLI RDVWGIEGPI DAAFTRINCQ GKTYLFKGSQ YWRFEDGVLD PDYPRNISDG FDGIPDNVDA ALALPAHSYS GRERVYFFKG KQYWEYQFQH QPSQEECEGS SLSAVFEHFA MMQRDSWEDI FELLFWGRTS AGTRQPQFIS RDWHGVPGQV DAAMAGRIYI SGMAPRPSLA KKQRFRHRNR KGYRSQRGHS RGRNQNSRRP SRATWLSLFS SEESNLGANN YDDYRMDWLV PATCEPIQSV FFFSGDKYYR VNLRTRRVDT VDPPYPRSIA QYWLGCPAPG HL