Recombinant HCV NS5a protein
NS5A is a component of the replication complex involved in RNA-binding. Its interaction with Human VAPB may target the viral replication complex to vesicles. This protein down-regulates viral IRES translation initiation and mediates interferon resistance, presumably by interacting with and inhibiting human EIF2AK2/PKR. NS5a seems to inhibit apoptosis by interacting with BIN1 and FKBP8. The hyperphosphorylated form of NS5A is an inhibitor of viral replication. Recombinant HCV (Hepatitis C Virus) NS5A protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Polyprotein, Hepatitis C virus non-structural 5a protein membrane anchor
Expression System
E.coli
Antigen Species
HCV
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
13.4 kDa (118aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Taylor D.R., et al. (2001) J. Virol. 75:1265-1273; ; Kalliampakou K.I., et al. (2015) J. Gen. Virol. 86:1015-1025
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 40% glycerol, 1mM DTT
Scientific Category
Virus
NCBI Accession Number
NP_671491
Species
HCV
AA Sequence
MGSSHHHHHH SSGLVPRGSH MSLKATCTAN HDSPDAELIE ANLLWRQEMG GNITRVESENKVVILDSFDP LVAEEDEREV SVPAEILRKS RRFARALPVW ARPDYNPPLV ETWKKPDY
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