Recombinant human SPECC1 protein

SPECC1, also known as Cytospin-B, belongs to the cytospin-A family. This protein is involved in cytokinesis and spindle organization. It may play a role in actin cytoskeleton organization and microtubule stabilization and hence required for proper cell adhesion and migration. Recombinant human SPECC1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Cytospin-B, CYTSB, HCMOGT-1, HCMOGT1, NSP

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 80% by SDS-PAGE

Molecular Weight

37.7 kDa (327aa) confirmed by MALDI-TOF

Additionnal Information

SPECC1, Cytospin-B, CYTSB, HCMOGT-1, HCMOGT1, NSP, ATGP2733-10 µg, ATGP2733-20 µg, ATGP2733-50 µg, ATGP2733-100 µg, ATGP2733-250 µg, ATGP2733-500 µg, ATGP2733-1 mg, ATGP2733-10, ATGP2733-20, ATGP2733-50, ATGP2733-100, ATGP2733-250, ATGP2733-500, ATGP2733-1

References & Citations

Sang N. et al. (2004) Oncogene. 23:9438-9446.; ; D'Agostino L. et al. (2008) Cell Cycle. 7:1810-1827

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 1mM DTT, 0.1M NaCl

Scientific Category

Cell Cycle

NCBI Accession Number

NP_690868

Uniprot Accession Number

Q5M775

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSTQEGKII ELEQKCTGIL EQGRFEREKL LNIQQQLTCS LRKVEEENQG ALEMIKRLKE ENEKLNEFLE LERHNNNMMA KTLEECRVTL EGLKMENGSL KSHLQGEKQK ATEASAVEQT AESCEVQEML KVARAEKDLL ELSCNELRQE LLKANGEIKH VSSLLAKVEK DYSYLKEICD HQAEQLSRTS LKLQEKASES DAEIKDMKET IFELEDQVEQ HRAVKLHNNQ LISELESSVI KLEEQKSDLE RQLKTLTKQM KEETEEWRRF QADLQTAVVV ANDIKCEAQQ ELRTVKRKLL EEEEKNA