Recombinant human HLA-F protein

HLA class I histocompatibility antigen, alpha chain F isoform 1 precursor, also known as HLA-F, is member of the MHC family that are specifically involved in the presentation of antigens to the T cell receptor. There are two classes of HLA antigens. HLA-F, belong to the Class I molecules. Class I molecules are expressed in nearly all cells and play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum. Recombinant human HLA-F, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

HLA class I histocompatibility antigen alpha chain F isoform 1, HLA class I histocompatibility antigen, alpha chain F isoform 1, CDA12, HLA-5.4, HLA-CDA12, HLAF

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

35.1 kDa (307aa) confirmed by MALDI-TOF

Additionnal Information

HLA-F, HLA class I histocompatibility antigen alpha chain F isoform 1, HLA class I histocompatibility antigen, alpha chain F isoform 1, CDA12, HLA-5.4, HLA-CDA12, HLAF, ATGP2732-10 µg, ATGP2732-20 µg, ATGP2732-50 µg, ATGP2732-100 µg, ATGP2732-250 µg, ATGP2732-500 µg, ATGP2732-1 mg, ATGP2732-10, ATGP2732-20, ATGP2732-50, ATGP2732-100, ATGP2732-250, ATGP2732-500, ATGP2732-1

References & Citations

Kropshofer H., et al. (1998) EMBO J. 17: 2971-2981.; ; Brunet A., et al. (2000) J Biol Chem. 275: 37062-37071.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT

Scientific Category

Immunology

NCBI Accession Number

NP_001091949.1

Uniprot Accession Number

P30511

Species

Human

AA Sequence

GSHSLRY FSTAVSRPGR GEPRYIAVEY VDDTQFLRFD SDAAIPRMEP REPWVEQEGP QYWEWTTGYA KANAQTDRVA LRNLLRRYNQ SEAGSHTLQG MNGCDMGPDG RLLRGYHQHA YDGKDYISLN EDLRSWTAAD TVAQITQRFY EAEEYAEEFR TYLEGECLEL LRRYLENGKE TLQRADPPKA HVAHHPISDH EATLRCWALG FYPAEITLTW QRDGEEQTQD TELVETRPAG DGTFQKWAAV VVPPGEEQRY TCHVQHEGLP QPLILRWEQS PQPTIPI