Recombinant human LMX1B protein

LMX1B is a member of LIM-homeodomain family of proteins containing two N-terminal zinc-binding LIM domains, 1 homeodomain, and a C-terminal glutamine-rich domain. It functions as a transcription factor, and is essential for the normal development of dorsal limb structures, the glomerular basement membrane, the anterior segment of the eye, and dopaminergic and serotonergic neurons. Mutations in this gene are associated with nail-patella syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. Recombinant human LMX1B protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

LIM homeobox transcription factor 1-beta isoform 1, LIM homeobox transcription factor 1 beta, LMX1.2, NPS1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 80% by SDS-PAGE

Molecular Weight

46.5 kDa (418aa)

Additionnal Information

LMX1B, LIM homeobox transcription factor 1-beta isoform 1, LIM homeobox transcription factor 1 beta, LMX1.2, NPS1, ATGP2730-10 µg, ATGP2730-20 µg, ATGP2730-50 µg, ATGP2730-100 µg, ATGP2730-250 µg, ATGP2730-500 µg, ATGP2730-1 mg, ATGP2730-10, ATGP2730-20, ATGP2730-50, ATGP2730-100, ATGP2730-250, ATGP2730-500, ATGP2730-1

References & Citations

Vollrath D., Jaramillo-babb V L., et al. (1998) . Hum. Mol. Genet. 7:1091-1098

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.4M urea

Scientific Category

Neuroscience

NCBI Accession Number

NP_002307

Uniprot Accession Number

O60663

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMDIATGP ESLERCFPRG QTDCAKMLDG IKMEEHALRP GPATLGVLLG SDCPHPAVCE GCQRPISDRF LMRVNESSWH EECLQCAACQ QALTTSCYFR DRKLYCKQDY QQLFAAKCSG CMEKIAPTEF VMRALECVYH LGCFCCCVCE RQLRKGDEFV LKEGQLLCKG DYEKEKDLLS SVSPDESDSV KSEDEDGDMK PAKGQGSQSK GSGDDGKDPR RPKRPRTILT TQQRRAFKAS FEVSSKPCRK VRETLAAETG LSVRVVQVWF QNQRAKMKKL ARRHQQQQEQ QNSQRLGQEV LSSRMEGMMA SYTPLAPPQQ QIVAMEQSPY GSSDPFQQGL TPPQMPGNDS IFHDIDSDTS LTSLSDCFLG SSDVGSLQAR VGNPIDRLYS MQSSYFAS