Recombinant human UBE2Z protein

UBE2z belongs to the ubiquitin-conjugating enzyme family. This protein catalyzes the covalent attachment of ubiquitin to other proteins. It is a specific substrate for UBA6, not charged with ubiquitin by UBE1 and may be involved in apoptosis regulation. Recombinant human UBE2Z protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Ubiquitin-conjugating enzyme E2 Z, E2 ubiquitin-conjugating enzyme Z, Uba6-specific E2 conjugating enzyme 1, Use1, Ubiquitin carrier protein Z, Ubiquitin-protein ligase Z, HOYS7

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

30.5 kDa (269aa) confirmed by MALDI-TOF

Additionnal Information

Use1, UBE2Z, ubiquitin-conjugating enzyme E2Z, HOYS7, uSE1, ATGP2726-10 µg, ATGP2726-20 µg, ATGP2726-50 µg, ATGP2726-100 µg, ATGP2726-250 µg, ATGP2726-500 µg, ATGP2726-1 mg, ATGP2726-10, ATGP2726-20, ATGP2726-50, ATGP2726-100, ATGP2726-250, ATGP2726-500, ATGP2726-1

References & Citations

Gu X, Zhao F., et al. (2007) Mol Biol Rep. 34 (3) :183-8

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT

Scientific Category

Ubiquitination

NCBI Accession Number

AAH15890

Uniprot Accession Number

Q9H832

Species

Human

AA Sequence

MSIYKEP PPGMFVVPDT VDMTKIHALI TGPFDTPYEG GFFLFVFRCP PDYPIHPPRV KLMTTGNNTV RFNPNFYRNG KVCLSILGTW TGPAWSPAQS ISSVLISIQS LMTENPYHNE PGFEQERHPG DSKNYNECIR HETIRVAVCD MMEGKCPCPE PLRGVMEKSF LEYYDFYEVA CKDRLHLQGQ TMQDPFGEKR GHFDYQSLLM RLGLIRQKVL ERLHNENAEM DSDSSSSGTE TDLHGSLRV