Recombinant human RNA polymerase II subunit RPB3/POLR2C protein

POLR2C is the third largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a cysteine rich region and exists as a heterodimer with another polymerase subunit, POLR2J. These two subunits form a core subassembly unit of the polymerase. A pseudogene has been identified on chromosome 21. Recombinant human POLR2C protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

DNA-directed RNA polymerase II subunit RPB3, hRPB33, hsRPB3, RPB3, RPB31

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

33.8 kDa (298aa) confirmed by MALDI-TOF

Additionnal Information

POLR2C, DNA-directed RNA polymerase II subunit RPB3, hRPB33, hsRPB3, RPB3, RPB31, ATGP2724-10 µg, ATGP2724-20 µg, ATGP2724-50 µg, ATGP2724-100 µg, ATGP2724-250 µg, ATGP2724-500 µg, ATGP2724-1 mg, ATGP2724-10, ATGP2724-20, ATGP2724-50, ATGP2724-100, ATGP2724-250, ATGP2724-500, ATGP2724-1

References & Citations

Bertolotti, A. et al. (1998) Mol. Cell. Biol. 18:1489-1497.; ; Acker, J. et al. (1998) J. Biol. Chem. 272:16815-16821

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.1M NaCl

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_116558

Uniprot Accession Number

P19387

Species

Human

AA Sequence

MPYANQP TVRITELTDE NVKFIIENTD LAVANSIRRV FIAEVPIIAI DWVQIDANSS VLHDEFIAHR LGLIPLISDD IVDKLQYSRD CTCEEFCPEC SVEFTLDVRC NEDQTRHVTS RDLISNSPRV IPVTSRNRDN DPNDYVEQDD ILIVKLRKGQ ELRLRAYAKK GFGKEHAKWN PTAGVAFEYD PDNALRHTVY PKPEEWPKSE YSELDEDESQ APYDPNGKPE RFYYNVESCG SLRPETIVLS ALSGLKKKLS DLQTQLSHEI QSDVLTIN