Recombinant human RNA polymerase II subunit RPB3/POLR2C protein
POLR2C is the third largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a cysteine rich region and exists as a heterodimer with another polymerase subunit, POLR2J. These two subunits form a core subassembly unit of the polymerase. A pseudogene has been identified on chromosome 21. Recombinant human POLR2C protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
DNA-directed RNA polymerase II subunit RPB3, hRPB33, hsRPB3, RPB3, RPB31
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
33.8 kDa (298aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Bertolotti, A. et al. (1998) Mol. Cell. Biol. 18:1489-1497.; ; Acker, J. et al. (1998) J. Biol. Chem. 272:16815-16821
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.1M NaCl
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
NP_116558
Uniprot Accession Number
P19387
Species
Human
AA Sequence
Frequently Asked Questions
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