Recombinant human EXOSC1 protein

EXOSC1 is a core component of the exosome. The mammalian exosome is required for rapid degradation of Au rich element-containing RNAs but not for poly (A) shortening. The association of this protein with the exosome is mediated by protein-protein interactions with ribosomal RNA-processing protein 42 and ribosomal RNA-processing protein 46. Recombinant human EXOSC1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Exosome complex component CSL4, Exosome component 1, CGI-108, CSL4, Csl4p, hCsl4p, p13, RP11-452K12.9, SKI4, Ski4p

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

23.8 kDa (218aa) confirmed by MALDI-TOF

Additionnal Information

EXOSC1, Exosome complex component CSL4, Exosome component 1, CGI-108, CSL4, Csl4p, hCsl4p, p13, RP11-452K12.9, SKI4, Ski4p, ATGP2723-10 µg, ATGP2723-20 µg, ATGP2723-50 µg, ATGP2723-100 µg, ATGP2723-250 µg, ATGP2723-500 µg, ATGP2723-1 mg, ATGP2723-10, ATGP2723-20, ATGP2723-50, ATGP2723-100, ATGP2723-250, ATGP2723-500, ATGP2723-1

References & Citations

Lai C.-H., Chou C.-Y., et al. (2000) Genome Res. 10:703-713

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_057130

Uniprot Accession Number

Q9Y3B2

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMAPPVRY CIPGERLCNL EEGSPGSGTY TRHGYIFSSL AGCLMKSSEN GALPVVSVVR ETESQLLPDV GAIVTCKVSS INSRFAKVHI LYVGSMPLKN SFRGTIRKED VRATEKDKVE IYKSFRPGDI VLAKVISLGD AQSNYLLTTA ENELGVVVAH SESGIQMVPI SWCEMQCPKT HTKEFRKVAR VQPEFLQT