Recombinant human DDX39A protein

DDX39A is a member of the DEAD box protein family. This protein is characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD) and are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of the DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. Alternatively spliced transcript variants encoding different isoforms have been found. Recombinant human DDX39A protein, fused to His-tag at N-terminus, was expressed in E. coli

Product Specifications

Product Name Alternative

ATP-dependent RNA helicase DDX39A, DEAD (Asp-Glu-Ala-Asp) box polypeptide 39A, BAT1, BAT1L, DDX39, DDXL, uRH49

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 80% by SDS-PAGE

Molecular Weight

31.0 kDa (274aa)

Additionnal Information

DDX39A, ATP-dependent RNA helicase DDX39A, DEAD (Asp-Glu-Ala-Asp) box polypeptide 39A, BAT1, BAT1L, DDX39, DDXL, uRH49, ATGP2719-10 µg, ATGP2719-20 µg, ATGP2719-50 µg, ATGP2719-100 µg, ATGP2719-250 µg, ATGP2719-500 µg, ATGP2719-1 mg, ATGP2719-10, ATGP2719-20, ATGP2719-50, ATGP2719-100, ATGP2719-250, ATGP2719-500, ATGP2719-1

References & Citations

Pryor A., Tung L. et al. (2004) . Nucleic Acids Res. 32:1857-1865

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.4M urea

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_005795

Uniprot Accession Number

O00148

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSEFMAEQD VENDLLDYDE EEEPQAPQES TPAPPKKDIK GSYVSIHSSG FRDFLLKPEL LRAIVDCGFE HPSEVQHECI PQAILGMDVL CQAKSGMGKT AVFVLATLQQ IEPVNGQVTV LVMCHTRELA FQISKEYERF SKYMPSVKVS VFFGGLSIKK DEEVLKKNCP HVVVGTPGRI LALVRNRSFS LKNVKHFVLD ECDKMLEQLD MRRDVQEIFR LTPHEKQCMM FSATLSKDIR PVCRKFMQDP MEVF