Recombinant human COA4 protein
Cytochrome c oxidase assembly factor 4 homolog, mitochondrial, also known as COA4, is the last enzyme of the mitochondrial respiratory chain, is a very complicated process and requires the assistance of a large number of accessory factors. It is essential for oxidative phosphorylation and comprises multiple complexes, including cytochrome c oxidase, assembled in macromolecular supercomplexes. Defects in COX4 assembly alter cellular respiration and produce severe human encephalomyopathies. Recombinant human COA4 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Cytochrome c oxidase assembly factor 4 homolog mitochondrial, Cytochrome c oxidase assembly factor 4 homolog, mitochondrial, CHCHD8, CMC3, E2IG2
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
12.5 kDa (110aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Diaz F., et al. (2012) Hum Mol Genet. 1 (23) : 5066-77.; ; Huigsloot M., et al. (2011) Am J Hum Genet. 8 (4) : 488-93.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT
Scientific Category
Other
NCBI Accession Number
NP_057649
Uniprot Accession Number
Q9NYJ1
Species
Human
AA Sequence
MSTSVPQ GHTWTQRVKK DDEEEDPLDQ LISRSGCAAS HFAVQECMAQ HQDWRQCQPQ VQAFKDCMSE QQARRQEELQ RRQEQAGAHH
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
Eukaryotic Translation Initiation Factor 2B, epsilon, phosphorylated (Ser539) (eIF2Be)
Eukaryotic Translation Initiation Factor 2B, epsilon, phosphorylated (Ser539) (eIF2Be)