Recombinant human GNRH1 protein
GNRH1 is secreted and then cleaved to form the 10 aa luteinizing hormone-releasing hormone (LHRH, also known as gonadoliberin-1), and prolactin release-inhibiting factor (also known as GnRH-associated peptide 1) . GNRH1 stimulates the release of luteinizing and follicle stimulating hormones, which are important for reproduction. The protein stimulates the secretion of gonadotropins; it stimulates the secretion of both luteinizing and follicle-stimulating hormones. Recombinant human GNRH1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Progonadoliberin-1 isoform 2 preproprotein, Gonadotropin-releasing hormone 1, GNRH, GRH, HH12, LHRH, LNRH
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
10.3 kDa (92aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Hayflick J.S., Adelman J.P., et al. (1989) Nucleic Acids Res. 17:6403-6403
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT
Scientific Category
Hormones & Cytokines
NCBI Accession Number
NP_001076580
Uniprot Accession Number
P01148
Species
Human
AA Sequence
MGSSHHHHHH SSGLVPRGSH MGSQHWSYGL RPGGKRDAEN LIDSFQEIVK EVGQLAETQR FECTTHQPRS PLRDLKGALE SLIEEETGQK KI
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