Recombinant human ZWILCH protein

ZWILCH belongs to the ZWILCH family. This protein is an essential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis. It is required for the assembly of the dynein-dynactin and MAD1-MAD2 complexes onto kinetochores. Recombinant human ZWILCH protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Protein zwilch homolog, hZwilch, KNTC1AP

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

69.6 kDa (614aa)

Additionnal Information

ZWILCH, Protein zwilch homolog, hZwilch, KNTC1AP, ATGP2709-10 µg, ATGP2709-20 µg, ATGP2709-50 µg, ATGP2709-100 µg, ATGP2709-250 µg, ATGP2709-500 µg, ATGP2709-1 mg, ATGP2709-10, ATGP2709-20, ATGP2709-50, ATGP2709-100, ATGP2709-250, ATGP2709-500, ATGP2709-1

References & Citations

Wang, C., et al. (2008) . Biol. Chem. 283 (17), 11565-11574; ; Meng, F., et al. (2003) Brain Res. 967 (1-2), 161-169

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 20% glycerol, 1mM DTT

Scientific Category

Cell Cycle

NCBI Accession Number

NP_060445

Uniprot Accession Number

Q9H900

Species

Human

AA Sequence

MWERLNC AAEDFYSRLL QKFNEEKKGI RKDPFLYEAD VQVQLISKGQ PNPLKNILNE NDIVFIVEKV PLEKEETSHI EELQSEETAI SDFSTGENVG PLALPVGKAR QLIGLYTMAH NPNMTHLKIN LPVTALPPLW VRCDSSDPEG TCWLGAELIT TNNSITGIVL YVVSCKADKN YSVNLENLKN LHKKRHHLST VTSKGFAQYE LFKSSALDDT ITASQTAIAL DISWSPVDEI LQIPPLSSTA TLNIKVESGE PRGPLNHLYR ELKFLLVLAD GLRTGVTEWL EPLEAKSAVE LVQEFLNDLN KLDGFGDSTK KDTEVETLKH DTAAVDRSVK RLFKVRSDLD FAEQLWCKMS SSVISYQDLV KCFTLIIQSL QRGDIQPWLH SGSNSLLSKL IHQSYHGTMD TVSLSGTIPV QMLLEIGLDK LKKDYISFFI GQELASLNHL EYFIAPSVDI QEQVYRVQKL HHILEILVSC MPFIKSQHEL LFSLTQICIK YYKQNPLDEQ HIFQLPVRPT AVKNLYQSEK PQKWRVEIYS GQKKIKTVWQ LSDSSPIDHL NFHKPDFSEL TLNGSLEERI FFTNMVTCSQ VHFK