Recombinant human EXOSC3 protein

EXOSC3 is a non-catalytic component of the human exosome, a complex with 3'-5' exoribonuclease activity that plays a role in numerous RNA processing and degradation activities. Related pseudogenes of this gene are found on chromosome 19 and 21. Alternatively spliced transcript variants encoding different isoforms have been described. Recombinant human EXOSC3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Exosome complex component RRP40 isoform 1, bA3J10.7, CGI-102, hRrp-40, p10, PCH1B, RP11-3J10.8, RRP40, Rrp40p

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

32.0 kDa (298aa) confirmed by MALDI-TOF

Additionnal Information

EXOSC3, exosome complex component RRP40 isoform 1, bA3J10.7, CGI-102, hRrp-40, p10, PCH1B, RP11-3J10.8, RRP40, Rrp40p, ATGP2703-10 µg, ATGP2703-20 µg, ATGP2703-50 µg, ATGP2703-100 µg, ATGP2703-250 µg, ATGP2703-500 µg, ATGP2703-1 mg, ATGP2703-10, ATGP2703-20, ATGP2703-50, ATGP2703-100, ATGP2703-250, ATGP2703-500, ATGP2703-1

References & Citations

Mukherjee D., et al (2002) . EMBO J. 21:165-174; ; van Dijk E.L., et al (2007) . RNA 13:1027-1035;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 0.15M NaCl, 30% glycerol, 1mM DTT

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_057126

Uniprot Accession Number

Q9NQT5

Species

Human

AA Sequence

MAEPASV AAESLAGSRA RAARTVLGQV VLPGEELLLP EQEDAEGPGG AVERPLSLNA RACSRVRVVC GPGLRRCGDR LLVTKCGRLR HKEPGSGSGG GVYWVDSQQK RYVPVKGDHV IGIVTAKSGD IFKVDVGGSE PASLSYLSFE GATKRNRPNV QVGDLIYGQF VVANKDMEPE MVCIDSCGRA NGMGVIGQDG LLFKVTLGLI RKLLAPDCEI IQEVGKLYPL EIVFGMNGRI WVKAKTIQQT LILANILEAC EHMTSDQRKQ IFSRLAES