Recombinant human PKNOX1 protein

Hmeobox protein PKNOX1 is a DNA-binding protein that forms stable complexes with PBX proteins which synergize with AP-1 binding factors to augment transcription of the urokinase gene. Also referred to as uEF3, PRP-1 or p64, PKNOX1 appears to be a general DNA-binding factor involved in modulating the transcriptional activity of AP-1 containing promoters. Recombinant human PKNOX1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Hmeobox protein PKNOX1, pkonx1c, PREP1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 80% by SDS-PAGE

Molecular Weight

49.7 kDa (456aa) confirmed by MALDI-TOF

Additionnal Information

PKNOX1, Hmeobox protein PKNOX1, pkonx1c, PREP1, ATGP2701-10 µg, ATGP2701-20 µg, ATGP2701-50 µg, ATGP2701-100 µg, ATGP2701-250 µg, ATGP2701-500 µg, ATGP2701-1 mg, ATGP2701-10, ATGP2701-20, ATGP2701-50, ATGP2701-100, ATGP2701-250, ATGP2701-500, ATGP2701-1

References & Citations

Nourse J., et al. (1990) Cell. 60: 535-545.; ; Monica K P., et al. (1991) Mol Cell Biol. 11: 6149-6157.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_004562

Uniprot Accession Number

P55347

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MMATQTLSID SYQDGQQMQV VTELKTEQDP NCSEPDAEGV SPPPVESQTP MDVDKQAIYR HPLFPLLALL FEKCEQSTQG SEGTTSASFD VDIENFVRKQ EKEGKPFFCE DPETDNLMVK AIQVLRIHLL ELEKVNELCK DFCSRYIACL KTKMNSETLL SGEPGSPYSP VQSQQIQSAI TGTISPQGIV VPASALQQGN VAMATVAGGT VYQPVTVVTP QGQVVTQTLS PGTIRIQNSQ LQLQLNQDLS ILHQDDGSSK NKRGVLPKHA TNVMRSWLFQ HIGHPYPTED EKKQIAAQTN LTLLQVNNWF INARRRILQP MLDSSCSETP KTKKKTAQNR PVQRFWPDSI ASGVAQPPPS ELTMSEGAVV TITTPVNMNV DSLQSLSSDG ATLAVQQVMM AGQSEDESVD STEEDAGALA PAHISGLVLE NSDSLQ